The Seven speedster A-Train, played by Jessie T. Usher, has a cameo on the series premiere of “Gen V.”

Jessie T. Usher as A-Train in the first trailer for “Gen V.” Prime Video
A-Train’s appearance on “Gen V” makes a lot of sense, since he’s an alum of Godolkin University.

The opening scene of “Gen V,” set years prior to the start of the series, shows the God U alum getting drafted for The Seven.

Elisabeth Shue reprises her role as Madelyn Stillwell.

Elisabeth Shue as Madelyn Stillwell on season one, episode one of “Gen V.” Prine
Stillwell was killed by Homelander on season one of “The Boys.”

However, Shue returns as Vought’s senior vice president of hero management in the opening flashback scene.

During her appearance, Madelyn talks about how “thrilled” she is to welcome A-Train into The Seven and brags about how they live in a post-racism world.

Colby Minifie pops up as Ashley Barrett, Vought’s stressed-out CEO.

Colby Minifie as Ashley Barrett on season one of “Gen V.” Prime Video
In a post-credits scene of episode one, Ashley reacts to the shocking death of Luke Riordan/Golden Boy in a rehearsed video and emphasizes that, despite the incident, God U is still a safe campus and the tragedy isn’t a reflection of Vought.

Ashley also appears on episode two, when she’s seen on the phone with God U’s dean, Indira Shetty, as well as the college’s trustees, discussing the fallout of Luke’s death.

She has an additional appearance on the finale.

P.J. Byrne returns as Adam Bourke, the director of the Vought Studios movie “Dawn of the Seven.”

PJ Byrne as Adam Bourke on season one of “Gen V.” Brooke Palmer/Prime Video
He teaches a class at God U that’s centered on acting for auditions.

Chace Crawford’s dim-witted supe, The Deep, makes an appearance in the form of a video message.

Chace Crawford as The Deep on season one, episode three of “Gen V.” Prime Video
His appearance on “Gen V” was first confirmed by Entertainment Weekly in early September.

On episode three, the God U alum raves about late professor Rich “Brink” Brinkerhoff in a video tribute.

“Brink didn’t just hone my skills,” The Deep says. “He sharpened my mind. He turned me into the curious, intelligent man that I am today.”

Jensen Ackles has a cameo as Soldier Boy on episode six.

Jensen Ackles as Soldier Boy on season one, episode six of “Gen V.” Prime Video
Ackles first teased his cameo on “Gen V” during an appearance at a convention in Louisiana in April, telling fans: “Soldier Boy pops up for a brief moment in this new spin-off of ‘The Boys.’ In fact, there’s a few cameos from the mothership that come into this spin-off. My little bit there was heavily [improvised].”

In September, EW shared a first-look image at the “Supernatural” star suited up as Soldier Boy on a forthcoming episode of “Gen V.”

During episode six, Soldier Boy briefly appears as Cate Dunlap’s imaginary friend during a scene that takes place in her mind.

Grace Mallory, played by Laila Robins, meets with Dean Shetty during episode seven.

Laila Robins as Grace Mallory on season one, episode seven of “Gen V.” Prime Video
In her conversation with Shetty, Mallory warns her against wanting to eliminate all supes using a virus that was created in The Woods.

Mallory compares Shetty to Billy Butcher, who is similarly consumed by a rage and hatred of supes.

Claudia Doumit returns as Victoria Neuman, a politician who, unbeknownst to most of the world, is a head-popping supe.

Claudia Doumit as Victoria Neuman on season one, episode seven of “Gen V.” Brooke Palmer/Prime Video
She visits God U for a town hall that turns into a riot during episode seven. Victoria also reveals that she and Marie share the same blood-manipulating powers.

Vought News Network anchor Cameron Coleman (Matthew Edison) appears on episode seven.

Matthew Edison as Cameron Coleman on season one, episode seven of “Gen V.” Prime Video
He interviews Polarity and Victoria Neuman.

Ashley Barrett’s assistant, known as Also Ashley (Sabrina Saudin), appears on the finale.

Sabrina Saudin as Also Ashley on season one, episode eight of “Gen V.” Prime Video
The two Ashleys, along with the rest of the God U trustees, meet to select a student to join The Seven as a way to distract from the school’s recent bad publicity.

Antony Starr reprises his role as Homelander.

Antony Starr as Homelander on season one, episode eight of “Gen V.” Prime Video
After Cate and Sam free the supes who were trapped in The Woods, they wreak havoc on campus. To put an end to the destruction, Ashley has her assistant call in Homelander.

He’s visibly angry at Marie when he arrives on the scene and directs his laser blasts at her.

Homelander is last seen watching the news at Vought Tower, which reveals that the massacre at God U was pinned on Marie, Andre, Jordan, and Emma — while Sam and Cate are praised as the real heroes.

Butcher, played by Karl Urban, pops up in the finale’s end credits scene.

Karl Urban as Billy Butcher on season one, episode eight of “Gen V.” Prime Video
He’s seen investigating the now-abandoned Woods alone, with a flashlight.

Butcher’s appearance was teased during the prior episode when Mallory was shown talking to someone on the phone and telling them to keep an eye on Shetty.